Reprogramming

The iPS Core employs both lentiviral and transposon mediated transduction methods which can be used to routinely reprogram mouse embryonic fibroblasts (MEFs) and tail tip fibroblasts to a stem cell like state by over expressing Oct 4, Sox 2, Klf 4 and c-Myc. Using these methods, colonies of cells exhibiting stem cell like properties can be produced in as little as 2 weeks. These cells can be expanded into cell lines that maintain these attributes and display endogenous expression of pluripotency factors even after the cessation of transgene induction. 

True ES cells and completely reprogrammed iPS cells are capable of differentiating into tissues of all three germ lineages (i.e. ectoderm, mesoderm, and endoderm). Validating the pluripotent state, however, is a daunting task requiring numerous assays. One goal of the iPS Core is to establish methods to evaluate the potential of iPS cells, and determine how they compare to true ES cells. To accomplish this, directed in-vitro differentiation and a variety of protein and gene expression assays can be used to determine the potential of iPS cells. In addition, the iPS core has validated iPS lines by teratoma formation in SCID mice, and iPS lines generated in the core have been used to generate adult germline chimeras. 

Click on the services below for fees and description of services.

Specific Services:

iPS cell generation

• Validation of iPS and ES Cells

     - Alkaline phosphatase staining

     - Karyotype analysis

     - Embryoid body Assay

     - Teratoma formation assay




For  information email:  Chris Abratte