Welcome

About the Core Facility

Established in August 2009, the Stem Cell and Transgenic Core Facility is located in the Veterinary Research Tower T9-010.  The Core’s faculty Director is John Schimenti (Professor, Biomedical Sciences), and it is staffed by Christian Abratte and Robert Munroe.

The goal of the core is to provide researchers at Cornell and other institutions with a resource for the generation and utilization of genetically-modified mice (transgenic, knockout, and genome-edited), cryopreservation, and rederivation of mouse strains. To this end, the Facility offers many services on a partial cost recovery basis for Cornell affiliated users, and full cost recovery for out-of-state customers.

Transgenic Mouse Models

For generating CRISPR – modified mice, we perform injection of RNAs (Cas9 + sgRNA) and gene targeting templates via microinjection into fertilized mouse embryos.  If you are new to CRISPRs, we strongly urge that you consult us beforehand.

Cryopreservation and Rederivation

The core can freeze mouse sperm or embryos and store them for a fee. We will re-constitute mice from frozen sperm or embryos stored at Cornell or received from external sources.

We can also perform rederivation of mouse strains to remove pathogens and allow them to enter higher health status rooms.

Service Rates and Contact Information

 For the current list of service and rates, please see the form here

For consultation on your design, or to initiate a project, please contact  Rob Munroe (rjm69@cornell.edu) or Christian Abratte (ca258@cornell.edu)